pAAV-syn-DIO-axon-jGCaMP8f
(Plasmid #220531)

• Purpose: For Cre-dependent enriched expression of GCaMP8f in axons
• Depositing Lab: Lin Tian
• Publication: For Cre-dependent enriched expression of GCaMP8f in axons (unpublished)

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Axon-DIO-GCaMP8f
• Insert Size (bp): 1582
• Promoter: hSynapsin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AfeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GAAGGCGCGCTGACGTCACTCGCCG
• 3′ sequencing primer: CAAATTTTGTAATCCAGAGGTTGATT

Resource Information

• Supplemental Documents:
  • pAAV-syn-DIO-axon-jGCaMP8f-WPRE.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry