Fubi-ChR2-GFP
(Plasmid #22051)

• Purpose: Viral expression of ChR2-GFP for neuronal excitation
• Depositing Lab: Edward Boyden
• Publication: Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: Lois/Baltimore
• Backbone size w/o insert (bp): 9115
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ChR2-GFP
• Alt name: channelrhodopsin-2
• Species: C. reinhardtii
• Insert Size (bp): 1677
• Mutation: N/A
• GenBank ID: EU714030
• Promoter: UbC
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: 5' GCACCTTTTGAAATGTAATC 3'
• 3′ sequencing primer: 5' AGAATACCAGTCAATCTTTCAC 3'

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.