Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2
(Plasmid #220508)

• Purpose: TGFb-inducible fluorescent reporter (TIE:EGFP)
• Depositing Lab: Leonard Zon
• Publication: Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2 (unpublished)

Backbone

• Vector backbone: pDestTol2pA2
• Backbone size w/o insert (bp): 4427
• Total vector size (bp): 5698
• Vector type: Zebrafish expression (Tol2)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: TGFb inducible enhancer
• Alt name: TIE
• Species: H. sapiens (human)
• Insert Size (bp): 282

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13R

Gene/Insert 2

• Gene/Insert name: beta-globin minimal promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 848
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F

Gene/Insert 3

• Gene/Insert name: SV40 PolyA signal
• Insert Size (bp): 135

Cloning Information for Gene/Insert 3

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry