pUC18-LucFF
(Plasmid #220496)

• Purpose: To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.
• Depositing Lab: Sébastien Britton
• Publication: Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.03.574002. doi: 10.1101/2024.01.03.574002.

Backbone

• Vector backbone: pUC18
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 4860
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly Luciferase
• Alt name: Luciferin 4-monooxygenase from Photinus pyralis
• Species: Photinus pyralis
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SV40-F
• 3′ sequencing primer: BGH-R

Resource Information

• Supplemental Documents:
  • pUC18-LucFF.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Industry MTA
  • Luciferase Limited Use Label License for Distribution to Industry