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Addgene

pUC18-LucFF
(Plasmid #220496)

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Item Catalog # Description Quantity Price (USD)
Plasmid 220496 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4860
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Alt name
    Luciferin 4-monooxygenase from Photinus pyralis
  • Species
    Photinus pyralis
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SV40-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18-LucFF was a gift from Sébastien Britton (Addgene plasmid # 220496 ; http://n2t.net/addgene:220496 ; RRID:Addgene_220496)

  • For your References section:

    Identification of the main barriers to Ku accumulation in chromatin. Bossaert M, Moreno A, Peixoto A, Pillaire MJ, Chanut P, Frit P, Calsou P, Loparo JJ, Britton S. bioRxiv [Preprint]. 2024 Jan 4:2024.01.03.574002. doi: 10.1101/2024.01.03.574002. 10.1101/2024.01.03.574002 PubMed 38260538
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