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pgRNA-bacteria_speG_M15
(Plasmid #220488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pgRNA-bacteria
  • Vector type
    RNAi, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gspeG
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Other
  • Mutation
    none

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgRNA-bacteria_speG_M15 was a gift from Emily Balskus (Addgene plasmid # 220488 ; http://n2t.net/addgene:220488 ; RRID:Addgene_220488)

  • For your References section:

    A metabolomics pipeline highlights microbial metabolism in bloodstream infections. Mayers JR, Varon J, Zhou RR, Daniel-Ivad M, Beaulieu C, Bholse A, Glasser NR, Lichtenauer FM, Ng J, Vera MP, Huttenhower C, Perrella MA, Clish CB, Zhao SD, Baron RM, Balskus EP. Cell. 2024 Jun 11:S0092-8674(24)00579-8. doi: 10.1016/j.cell.2024.05.035. 10.1016/j.cell.2024.05.035 PubMed 38885650
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