pET28a_PA1472_Cterm_His
(Plasmid
#220485)
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Purposeheterologous expression of PA1472 for protein purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePA1472
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SpeciesP. aeruginosa
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Mutationnone
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Tag
/ Fusion Protein
- 6xHs (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a_PA1472_Cterm_His was a gift from Emily Balskus (Addgene plasmid # 220485 ; http://n2t.net/addgene:220485 ; RRID:Addgene_220485) -
For your References section:
A metabolomics pipeline highlights microbial metabolism in bloodstream infections. Mayers JR, Varon J, Zhou RR, Daniel-Ivad M, Beaulieu C, Bholse A, Glasser NR, Lichtenauer FM, Ng J, Vera MP, Huttenhower C, Perrella MA, Clish CB, Zhao SD, Baron RM, Balskus EP. Cell. 2024 Jun 11:S0092-8674(24)00579-8. doi: 10.1016/j.cell.2024.05.035. 10.1016/j.cell.2024.05.035 PubMed 38885650