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Addgene

pET28a_speG_Cterm_His
(Plasmid #220483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    speG
  • Species
    E. coli
  • Mutation
    none
  • Tag / Fusion Protein
    • 6xHs (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_speG_Cterm_His was a gift from Emily Balskus (Addgene plasmid # 220483 ; http://n2t.net/addgene:220483 ; RRID:Addgene_220483)

  • For your References section:

    A metabolomics pipeline highlights microbial metabolism in bloodstream infections. Mayers JR, Varon J, Zhou RR, Daniel-Ivad M, Beaulieu C, Bholse A, Glasser NR, Lichtenauer FM, Ng J, Vera MP, Huttenhower C, Perrella MA, Clish CB, Zhao SD, Baron RM, Balskus EP. Cell. 2024 Jun 11:S0092-8674(24)00579-8. doi: 10.1016/j.cell.2024.05.035. 10.1016/j.cell.2024.05.035 PubMed 38885650
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