CROPseq-MPSV-eBFP2
(Plasmid
#220472)
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Purpose(Empty Backbone) Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROPseq-Guide-Puro
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Backbone manufacturerChristoph Bock lab
- Backbone size (bp) 7820
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Modifications to backboneExchange of EF1a-Puromycin cassette with MPSV-eBFP2 cassette
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersEBFP2-fluorescence
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byWe received the CROPseq-Guide-Puro-vector from Paul Datlinger from Christoph Bocks Lab (CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences). Further, the MPSV-eBFP2-insert was received from Kristoffer Riecken from Boris Fehses lab (Departement of Stem cell Transplantation at the University Medical Center Hamburg-Eppendorf).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROPseq-MPSV-eBFP2 was a gift from Christian Krebs (Addgene plasmid # 220472 ; http://n2t.net/addgene:220472 ; RRID:Addgene_220472) -
For your References section:
Targeting T cell plasticity in kidney and gut inflammation by pooled single-cell CRISPR screening. Enk LUB, Hellmig M, Riecken K, Kilian C, Datlinger P, Jauch-Speer SL, Neben T, Sultana Z, Sivayoganathan V, Borchers A, Paust HJ, Zhao Y, Asada N, Liu S, Agalioti T, Pelczar P, Wiech T, Bock C, Huber TB, Huber S, Bonn S, Gagliani N, Fehse B, Panzer U, Krebs CF. Sci Immunol. 2024 Jun 14;9(96):eadd6774. doi: 10.1126/sciimmunol.add6774. Epub 2024 Jun 14. 10.1126/sciimmunol.add6774 PubMed 38875317
Map uploaded by the depositor.
