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Addgene

pSliceRep_B_miR-196a
(Plasmid #220470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBI-CMV1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6536
  • Modifications to backbone
    Added FLuc and IRES-NanoLuc-PEST degron
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUMO_Eu1
  • Species
    Synthetic
  • Insert Size (bp)
    426
  • Promoter Minimal CMV
  • Tag / Fusion Protein
    • 3xHA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.15.562437 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSliceRep_B_miR-196a was a gift from David Bartel (Addgene plasmid # 220470 ; http://n2t.net/addgene:220470 ; RRID:Addgene_220470)

  • For your References section:

    The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex. Wang PY, Bartel DP. Mol Cell. 2024 Jul 10:S1097-2765(24)00533-1. doi: 10.1016/j.molcel.2024.06.026. 10.1016/j.molcel.2024.06.026 PubMed 39025072
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