ChR2-2A-Halo
(Plasmid #22047)

• Purpose: Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity
• Depositing Lab: Edward Boyden
• Publication: Han et al Front Mol Neurosci. 2009 Aug 27:2:12. doi: 10.3389/neuro.02.012.2009

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 3963
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ChR2-GFP-2A-Halo-YFP
• Alt name: NpHR
• Alt name: channelrhodopsin
• Alt name: halorhodopsin
• Species: C. reinhardtii, N. pharaonis
• Insert Size (bp): 3356
• Mutation: Note that there is a S147R mut in YFP. This mutation is not known to affect fluorescence.
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP and YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: AGCAGAGCTGGTTTAGTGAA
• 3′ sequencing primer: TAAAACCTCTACAAATGTGG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.