psiTE(1)-Gal4
(Plasmid
#220450)
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PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCfaN-Gal4-CfaC
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SpeciesOther
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Insert Size (bp)3177
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psiTE(1)-Gal4 was a gift from Benjamin White (Addgene plasmid # 220450 ; http://n2t.net/addgene:220450 ; RRID:Addgene_220450) -
For your References section:
Hox gene-specific cellular targeting using split intein Trojan exons. Diao F, Vasudevan D, Heckscher ES, White BH. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2317083121. doi: 10.1073/pnas.2317083121. Epub 2024 Apr 11. 10.1073/pnas.2317083121 PubMed 38602904