psiT-GEM(0)
(Plasmid
#220446)
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PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCfaN-Gal4-CfaC
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SpeciesOther
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Insert Size (bp)3177
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: AgeI SphI NotI (unknown if destroyed), 3' Cloning Site: AscI KpnI SpeI (unknown if destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psiT-GEM(0) was a gift from Benjamin White (Addgene plasmid # 220446 ; http://n2t.net/addgene:220446 ; RRID:Addgene_220446) -
For your References section:
Hox gene-specific cellular targeting using split intein Trojan exons. Diao F, Vasudevan D, Heckscher ES, White BH. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2317083121. doi: 10.1073/pnas.2317083121. Epub 2024 Apr 11. 10.1073/pnas.2317083121 PubMed 38602904