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Addgene

RT-4
(Plasmid #220443)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220443 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II
  • Total vector size (bp) 4733
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PUF3
  • Alt name
    pBSII/PUF3 5’ flank/PUP2/3HA/URA3/PUF3 3’ flank
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1500
  • Entrez Gene
    PUF3 (a.k.a. YLL013C)
  • Tag / Fusion Protein
    • 3xHA

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RT-4 was a gift from Marvin Wickens (Addgene plasmid # 220443 ; http://n2t.net/addgene:220443 ; RRID:Addgene_220443)

  • For your References section:

    Protein-RNA networks revealed through covalent RNA marks. Lapointe CP, Wilinski D, Saunders HA, Wickens M. Nat Methods. 2015 Dec;12(12):1163-70. doi: 10.1038/nmeth.3651. Epub 2015 Nov 2. 10.1038/nmeth.3651 PubMed 26524240
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