CYLBL_Neo_CAGdCas9-KRAB
(Plasmid
#220439)
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PurposeCAG-dCas9-KRAB knock-in using TALEN at CLYBL locus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUCM-CLYBL-hNIL
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Backbone manufacturerMichael Ward (Addgene plasmid # 105841)
- Total vector size (bp) 13583
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Modifications to backbonereplaced insertion with CAG-dCas9-KRAB
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Vector typeMammalian Expression, CRISPR, TALEN
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Selectable markersNeomycin (select with G418) ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesSynthetic
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Insert Size (bp)7610
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MutationD10A and H840A
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Tag
/ Fusion Protein
- KRAB (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gcatggacgagtaaGGTACCataacttcgtatagcatacattatacgaagttataaaccc
- 3′ sequencing primer ggatgaggagatTTAATTAAacatgtaaggaaaattttagggatgttaaagaaaaaaataacac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CYLBL_Neo_CAGdCas9-KRAB was a gift from Yin Shen (Addgene plasmid # 220439 ; http://n2t.net/addgene:220439 ; RRID:Addgene_220439) -
For your References section:
Functional characterization of Alzheimer's disease genetic variants in microglia. Yang X, Wen J, Yang H, Jones IR, Zhu X, Liu W, Li B, Clelland CD, Luo W, Wong MY, Ren X, Cui X, Song M, Liu H, Chen C, Eng N, Ravichandran M, Sun Y, Lee D, Van Buren E, Jiang MZ, Chan CSY, Ye CJ, Perera RM, Gan L, Li Y, Shen Y. Nat Genet. 2023 Oct;55(10):1735-1744. doi: 10.1038/s41588-023-01506-8. Epub 2023 Sep 21. 10.1038/s41588-023-01506-8 PubMed 37735198
Map uploaded by the depositor.
