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CYLBL_Neo_CAGdCas9-KRAB
(Plasmid #220439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCM-CLYBL-hNIL
  • Backbone manufacturer
    Michael Ward (Addgene plasmid # 105841)
  • Total vector size (bp) 13583
  • Modifications to backbone
    replaced insertion with CAG-dCas9-KRAB
  • Vector type
    Mammalian Expression, CRISPR, TALEN
  • Selectable markers
    Neomycin (select with G418) ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Synthetic
  • Insert Size (bp)
    7610
  • Mutation
    D10A and H840A
  • Tag / Fusion Protein
    • KRAB (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcatggacgagtaaGGTACCataacttcgtatagcatacattatacgaagttataaaccc
  • 3′ sequencing primer ggatgaggagatTTAATTAAacatgtaaggaaaattttagggatgttaaagaaaaaaataacac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CYLBL_Neo_CAGdCas9-KRAB was a gift from Yin Shen (Addgene plasmid # 220439 ; http://n2t.net/addgene:220439 ; RRID:Addgene_220439)

  • For your References section:

    Functional characterization of Alzheimer's disease genetic variants in microglia. Yang X, Wen J, Yang H, Jones IR, Zhu X, Liu W, Li B, Clelland CD, Luo W, Wong MY, Ren X, Cui X, Song M, Liu H, Chen C, Eng N, Ravichandran M, Sun Y, Lee D, Van Buren E, Jiang MZ, Chan CSY, Ye CJ, Perera RM, Gan L, Li Y, Shen Y. Nat Genet. 2023 Oct;55(10):1735-1744. doi: 10.1038/s41588-023-01506-8. Epub 2023 Sep 21. 10.1038/s41588-023-01506-8 PubMed 37735198
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