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Addgene

pafG-CBE-3T
(Plasmid #220374)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220374 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    hAMA1-pyrG-AmpR-ori-2 micron-URA3
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    hA3A-nCas9
  • Promoter Ptef1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tRNA and gRNA scaffold
  • Promoter tRNA

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pafG-CBE-3T was a gift from Xue Gao (Addgene plasmid # 220374 ; http://n2t.net/addgene:220374 ; RRID:Addgene_220374)

  • For your References section:

    Multiplex Base-Editing Enables Combinatorial Epigenetic Regulation for Genome Mining of Fungal Natural Products. Zhao F, Sun C, Liu Z, Cabrera A, Escobar M, Huang S, Yuan Q, Nie Q, Luo KL, Lin A, Vanegas JA, Zhu T, Hilton IB, Gao X. J Am Chem Soc. 2023 Jan 11;145(1):413-421. doi: 10.1021/jacs.2c10211. Epub 2022 Dec 21. 10.1021/jacs.2c10211 PubMed 36542862
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