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Addgene

pBBR-Cas9ΔyqhD-HA500
(Plasmid #220369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1MCS2
  • Vector type
    Bacterial Expression, CRISPR ; Broad host range

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    storage in E. coli, and genome editing in P. putida
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    SpCas9
  • Species
    S. pyogenes

Gene/Insert 2

  • Gene/Insert name
    sgRNA for yqhD in P. putida KT2440

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBBR-Cas9ΔyqhD-HA500 was a gift from Yilan Liu (Addgene plasmid # 220369 ; http://n2t.net/addgene:220369 ; RRID:Addgene_220369)
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