pBBR-Cas9ΔyqhD-HA500
(Plasmid
#220369)
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PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1MCS2
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Vector typeBacterial Expression, CRISPR ; Broad host range
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsstorage in E. coli, and genome editing in P. putida
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameSpCas9
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SpeciesS. pyogenes
Gene/Insert 2
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Gene/Insert namesgRNA for yqhD in P. putida KT2440
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBBR-Cas9ΔyqhD-HA500 was a gift from Yilan Liu (Addgene plasmid # 220369 ; http://n2t.net/addgene:220369 ; RRID:Addgene_220369)
Map uploaded by the depositor.
