pSLIK DNM1L-V5 neo
(Plasmid
#220363)
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PurposeLentiviral expression vector for an inducible V5-tagged DNM1L construct
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSLIK neo
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Backbone manufacturerAddgene #25735
- Backbone size w/o insert (bp) 13286
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDNM1L
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Alt nameDRP1
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SpeciesH. sapiens (human)
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Insert Size (bp)2175
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Entrez GeneDNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLIK DNM1L-V5 neo was a gift from Kevin Janes (Addgene plasmid # 220363 ; http://n2t.net/addgene:220363 ; RRID:Addgene_220363)