MSCV-opEBNA2-IRES-mCherry
(Plasmid #220355)

• Purpose: Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)
• Depositing Lab: Klaus Rajewsky
• Publication: Zhang et al Front Immunol. 2023 Dec 19;14:1331730. doi: 10.3389/fimmu.2023.1331730. eCollection 2023.

Backbone

• Vector backbone: pMIGR1 (MSCV-IRES-GFP)
• Modifications to backbone: GFP replaced by mCherry (Nco I/Sal I)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: opEBNA2
• Alt name: expression-optimised EBNA2 coding sequence
• Alt name: expression-optimised Epstein-Barr Nuclear Antigen 2 coding sequence
• Species: Epstein-Barr Virus (EBV) strain B95-8
• Insert Size (bp): 1461
• Mutation: codon-optimised for expression in mammalian cells
• Entrez Gene: EBNA-2 (a.k.a. HHV4_EBNA-2)

Resource Information

• Supplemental Documents:
  • MSCV-opEBNA2-IRES-mCherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry