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MSCV-opEBNA2-IRES-mCherry
(Plasmid #220355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIGR1 (MSCV-IRES-GFP)
  • Modifications to backbone
    GFP replaced by mCherry (Nco I/Sal I)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    opEBNA2
  • Alt name
    expression-optimised EBNA2 coding sequence
  • Alt name
    expression-optimised Epstein-Barr Nuclear Antigen 2 coding sequence
  • Species
    Epstein-Barr Virus (EBV) strain B95-8
  • Insert Size (bp)
    1461
  • Mutation
    codon-optimised for expression in mammalian cells
  • Entrez Gene
    EBNA-2 (a.k.a. HHV4_EBNA-2)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-opEBNA2-IRES-mCherry was a gift from Klaus Rajewsky (Addgene plasmid # 220355 ; http://n2t.net/addgene:220355 ; RRID:Addgene_220355)

  • For your References section:

    LMP1 and EBNA2 constitute a minimal set of EBV genes for transformation of human B cells. Zhang J, Sommermann T, Li X, Gieselmann L, de la Rosa K, Stecklum M, Klein F, Kocks C, Rajewsky K. Front Immunol. 2023 Dec 19;14:1331730. doi: 10.3389/fimmu.2023.1331730. eCollection 2023. 10.3389/fimmu.2023.1331730 PubMed 38169736
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