MSCV-opEBNA2-IRES-GFP
(Plasmid #220354)

• Purpose: Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
• Depositing Lab: Klaus Rajewsky
• Publication: Zhang et al Front Immunol. 2023 Dec 19;14:1331730. doi: 10.3389/fimmu.2023.1331730. eCollection 2023.

Backbone

• Vector backbone: pMIGR1 (MSCV-IRES-GFP)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: opEBNA2
• Alt name: expression-optimised EBNA2 coding sequence
• Alt name: expression-optimised Epstein-Barr Nuclear Antigen 2 coding sequence
• Species: Epstein-Barr Virus (EBV) strain B95-8
• Insert Size (bp): 1488
• Mutation: codon-optimised for expression in mammalian cells
• Entrez Gene: EBNA-2 (a.k.a. HHV4_EBNA-2)
• Tag / Fusion Protein:
  • 3 x HA tag (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: MSCV-gag Forward 5-cccttgaacctcctcgttcgacc-3

Resource Information

• Supplemental Documents:
  • MSCV-opEBNA2-HA-IRES-GFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry