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pKI_ΩBBMVP16&WUS
(Plasmid #220348)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKI1.1R
  • Total vector size (bp) 14733
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    BABY BOOM
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    BBM (a.k.a. AT5G17430, BABY BOOM, T10B6.90, T10B6_90)
  • Promoter CaMV 35S
  • Tag / Fusion Protein
    • VP16 transcriptional activation domain (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    WUSCHEL
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    WUS (a.k.a. AT2G17950, PGA6, T27K22.18, T27K22_18, WUS1, WUSCHEL, WUSCHEL 1)
  • Promoter RPS5A

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKI_ΩBBMVP16&WUS was a gift from Tomoko Igawa (Addgene plasmid # 220348 ; http://n2t.net/addgene:220348 ; RRID:Addgene_220348)

  • For your References section:

    Autonomous differentiation of transgenic cells requiring no external hormone application: the endogenous gene expression and phytohormone behaviors. Sato Y, Minamikawa MF, Pratama BB, Koyama S, Kojima M, Takebayashi Y, Sakakibara H, Igawa T. Front Plant Sci. 2024 Apr 3;15:1308417. doi: 10.3389/fpls.2024.1308417. eCollection 2024. 10.3389/fpls.2024.1308417 PubMed 38633452
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