Pet28a-His-tag-BS2
(Plasmid
#220347)
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PurposeE. coli expression vector to purify BS2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepet28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBS2
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SpeciesBacillus subtilis
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Insert Size (bp)1535
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Tag
/ Fusion Protein
- His tag (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pet28a-His-tag-BS2 was a gift from Bryan Dickinson (Addgene plasmid # 220347 ; http://n2t.net/addgene:220347 ; RRID:Addgene_220347) -
For your References section:
Bioorthogonal masked acylating agents for proximity-dependent RNA labelling. Pani S, Qiu T, Kentala K, Azizi SA, Dickinson BC. Nat Chem. 2024 Apr 9. doi: 10.1038/s41557-024-01493-1. 10.1038/s41557-024-01493-1 PubMed 38594368
Map uploaded by the depositor.
