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Addgene

V5-BS2-NIK3x (Nucleolus)
(Plasmid #220346)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 220346 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    d0
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BS2
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    1633
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 tag (N terminal on insert)
    • Nik3x (Nucleolus localization tag) (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V5-BS2-NIK3x (Nucleolus) was a gift from Bryan Dickinson (Addgene plasmid # 220346 ; http://n2t.net/addgene:220346 ; RRID:Addgene_220346)
  • For your References section:

    Bioorthogonal masked acylating agents for proximity-dependent RNA labelling. Pani S, Qiu T, Kentala K, Azizi SA, Dickinson BC. Nat Chem. 2024 Apr 9. doi: 10.1038/s41557-024-01493-1. 10.1038/s41557-024-01493-1 PubMed 38594368