Skip to main content
Addgene

tRECs/3xUAS-FP attB
(Plasmid #220330)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUAST-3x attB
  • Backbone size w/o insert (bp) 10697
  • Total vector size (bp) 16869
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    grow at 32C in case of problems
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    B3RT-STOP-B3RT-TagBFP-3xHA
  • Species
    Synthetic
  • Promoter UAS

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer see plasmid sequence file
  • 3′ sequencing primer see plasmid sequence file
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    KDRT-STOP-KDRT-GFP-HSV
  • Species
    Synthetic
  • Promoter UAS

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer see plasmid sequence file
  • 3′ sequencing primer see plasmid sequence file
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    FRTm71-STOP-FRTm71-mCherry
  • Species
    Synthetic
  • Promoter UAS

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRi (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer see plasmid sequence file
  • 3′ sequencing primer see plasmid sequence file
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tRECs/3xUAS-FP attB was a gift from Erdem Bangi (Addgene plasmid # 220330 ; http://n2t.net/addgene:220330 ; RRID:Addgene_220330)
Related items: