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Addgene

hs-cre::EBD, UAS-dBrainbow attB
(Plasmid #220326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    2xUAS-1xhsp70-attB
  • Backbone size w/o insert (bp) 10765
  • Total vector size (bp) 17091
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    reduce growth temperature to 32C if there are problems with growth and transformation
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cre::EBD (with intron)
  • Species
    Synthetic
  • Insert Size (bp)
    2077
  • Promoter hs

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer see plasmid sequence file
  • 3′ sequencing primer see plasmid sequence file
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    dBrainbow
  • Species
    Synthetic
  • Insert Size (bp)
    4264
  • Promoter UAS

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer see plasmid sequence file
  • 3′ sequencing primer see plasmid sequence file
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hs-cre::EBD, UAS-dBrainbow attB was a gift from Erdem Bangi (Addgene plasmid # 220326 ; http://n2t.net/addgene:220326 ; RRID:Addgene_220326)
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