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Addgene

pLEX305_3xFLAG_RELA1
(Plasmid #220324)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX305-C-dTAG
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 9600
  • Total vector size (bp) 9516
  • Modifications to backbone
    Puromycin (PURO) selection marker was changed to Blastocidin (BSD)
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    24 hours at 30 degrees
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RELA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1653
  • Entrez Gene
    RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
  • Promoter hPGK
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX305_3xFLAG_RELA1 was a gift from Jonathan Brown (Addgene plasmid # 220324 ; http://n2t.net/addgene:220324 ; RRID:Addgene_220324)
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