pLEX305_3xFLAG_STAT1
(Plasmid #220323)

• Purpose: Expresses full length human STAT1 with an N-terminal 3xFLAG tag
• Depositing Lab: Jonathan Brown
• Publication: Transcriptional Synergy in Human Aortic Endothelial Cells is Vulnerable to Combination p300/CBP and BET bromodomain Inhibition (unpublished)

Backbone

• Vector backbone: pLEX305-C-dTAG
• Backbone manufacturer: n/a
• Backbone size w/o insert (bp): 9600
• Total vector size (bp): 10113
• Modifications to backbone: Puromycin (PURO) selection marker was changed to Blastocidin (BSD)
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: 24 hours at 30 degrees
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAT1 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 2343
• Entrez Gene: STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
• Promoter: PGK
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GTGTTCCGCATTCTGCAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry