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GFP-Bcl-2-K17R-K22R
(Plasmid #220294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEMD-C1
  • Backbone size w/o insert (bp) 4702
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    720
  • Mutation
    K17R-K22R
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)
  • Promoter CMV
  • Tag / Fusion Protein
    • Emerald GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Bcl-2-K17R-K22R was a gift from Xinlai Cheng (Addgene plasmid # 220294 ; http://n2t.net/addgene:220294 ; RRID:Addgene_220294)

  • For your References section:

    Thalidomide derivatives degrade BCL-2 by reprogramming the binding surface of CRBN. Wang J, Heinz M, Han K, Shah VJ, Hasselbeck S, Schwalm MP, Rathore R, Hummer G, Zhou J, Dikic I, Cheng X. Cell Reports Physical Science, Volume 5, Issue 5, 101960 10.1016/j.xcrp.2024.101960
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