GFP-Bcl-2-K22R
(Plasmid
#220293)
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Purposemammalian expression of Bcl-2-K22R tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEMD-C1
- Backbone size w/o insert (bp) 4702
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl2
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SpeciesH. sapiens (human)
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Insert Size (bp)720
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MutationK22R
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Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
- Promoter CMV
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Tag
/ Fusion Protein
- Emerald GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Bcl-2-K22R was a gift from Xinlai Cheng (Addgene plasmid # 220293 ; http://n2t.net/addgene:220293 ; RRID:Addgene_220293) -
For your References section:
Thalidomide derivatives degrade BCL-2 by reprogramming the binding surface of CRBN. Wang J, Heinz M, Han K, Shah VJ, Hasselbeck S, Schwalm MP, Rathore R, Hummer G, Zhou J, Dikic I, Cheng X. Cell Reports Physical Science, Volume 5, Issue 5, 101960 10.1016/j.xcrp.2024.101960