pTYC10_pPromALB_STARRseq
(Plasmid
#220289)
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Purpose(Empty Backbone) STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonehSTARR-seq_SCP1 vector
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Backbone manufacturerAlexander Stark (Addgene # 99292)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTYC10_pPromALB_STARRseq was a gift from David Waxman (Addgene plasmid # 220289 ; http://n2t.net/addgene:220289 ; RRID:Addgene_220289) -
For your References section:
HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo. Chang TY, Waxman DJ. BMC Genomics. 2024 Dec 24;25(1):1240. doi: 10.1186/s12864-024-11162-9. 10.1186/s12864-024-11162-9 PubMed 39716078
