pTYC2_pPromALB_enh29774_FireflyLuc
(Plasmid
#220286)
-
PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 220286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL4.10
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemouse liver DNase hypersensitive site # 29774
-
Alt nameall_tcpo_peak_29774, mm9 genome- chr19 : 39079577-39080487
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)910
- Promoter minimal albumin promoter
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a 1bp deletion in all_tcpo_peak_29774 that is unlikely to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTYC2_pPromALB_enh29774_FireflyLuc was a gift from David Waxman (Addgene plasmid # 220286 ; http://n2t.net/addgene:220286 ; RRID:Addgene_220286) -
For your References section:
HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo. Chang TY, Waxman DJ. BMC Genomics. 2024 Dec 24;25(1):1240. doi: 10.1186/s12864-024-11162-9. 10.1186/s12864-024-11162-9 PubMed 39716078