pGYL17_pPromALB_enhALB_FireflyLuc
(Plasmid
#220283)
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PurposeFirefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLuc
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.10
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAlb Enhancer
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Alt namePartial all_tcpo_peak_43997, chr5: 90,597,816-90,598,195 (C57BL/6J mouse genome GRCm39)
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SpeciesM. musculus (mouse)
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Insert Size (bp)380
- Promoter minimal albumin promoter
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGYL17_pPromALB_enhALB_FireflyLuc was a gift from David Waxman (Addgene plasmid # 220283 ; http://n2t.net/addgene:220283 ; RRID:Addgene_220283) -
For your References section:
HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo. Chang TY, Waxman DJ. BMC Genomics. 2024 Dec 24;25(1):1240. doi: 10.1186/s12864-024-11162-9. 10.1186/s12864-024-11162-9 PubMed 39716078