pHIV-EGFP.Flag-EZH2-mt2*
(Plasmid #220246)

• Purpose: Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.
• Depositing Lab: Chen Davidovich
• Publication: Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41588-024-01740-8.

Backbone

• Vector backbone: pHIV-EGFP
• Backbone manufacturer: addgene Plasmid #21373
• Backbone size w/o insert (bp): 7746
• Total vector size (bp): 9995
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EZH2 mt 2*
• Alt name: Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit mutant 2 asterisk
• Alt name: Histone-lysine N-methyltransferase
• Species: H. sapiens (human)
• Insert Size (bp): 2934
• Mutation: PRKKKR494-499NAAIRS
• GenBank ID: Q15910
• Entrez Gene: EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
• Promoter: EF-1α
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: 5'-TGGAATTTGCCCTTTTTGAG-3'
• 3′ sequencing primer: 5'-AGGAACTGCTTCCTTCACGA-3'

Resource Information

• Supplemental Documents:
  • EZH2_mt3_pHIVEGFP_1_04.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry