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pFB1.HMBP.PrS.EZH2-mt1
(Plasmid #220239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1 (pFB1.HMBP.A3.PrS.ybbR)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5986
  • Total vector size (bp) 8174
  • Modifications to backbone
    N-terminal His-MBP PreScission cleavage site
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EZH2 mt 1
  • Alt name
    Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit mutant 1
  • Alt name
    Histone-lysine N-methyltransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2934
  • Mutation
    R27A, R31A, F165A
  • GenBank ID
    Q15910
  • Entrez Gene
    EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6x His, MBP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB1.HMBP.PrS.EZH2-mt1 was a gift from Chen Davidovich (Addgene plasmid # 220239 ; http://n2t.net/addgene:220239 ; RRID:Addgene_220239)

  • For your References section:

    Inseparable RNA binding and chromatin modification activities of a nucleosome-interacting surface in EZH2. Gail EH, Healy E, Flanigan SF, Jones N, Ng XH, Uckelmann M, Levina V, Zhang Q, Davidovich C. Nat Genet. 2024 May 14. doi: 10.1038/s41588-024-01740-8. 10.1038/s41588-024-01740-8 PubMed 38744974
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