pET15-ZmPYK
(Plasmid #220230)

• Purpose: Inducible expression of Zymomonas mobilis pyruvate kinase
• Depositing Labs: Aron Fenton, Liskin Swint-Kruse
• Publication: Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad030. doi: 10.1093/database/baad030.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: EMD Millipore
• Backbone size w/o insert (bp): 5708
• Total vector size (bp): 7136
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Zymomonas mobilis pyruvate kinase
• Alt name: ZmPYK
• Species: Zymomonas mobilis
• Insert Size (bp): 1494
• Entrez Gene: pyk (a.k.a. ZCP4_RS05440, ZCP4_1090)
• Entrez Gene: ZMO0152 (a.k.a. ZMO0152)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-TEV (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pCDF-ZmPYK.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression and purification of an exogenous PYK requires the E. coli strain QTF60, a BL21-derivative lacking both endogenous PYK genes (pykF and pykA).