pet15b_ack
(Plasmid
#220229)
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PurposeBacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAcetate kinase
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Alt nameackA
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Insert Size (bp)1263
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Entrez GeneackA (a.k.a. b2296, ECK2290)
- Promoter T7
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Tag
/ Fusion Protein
- Histag (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7_prom
- 3′ sequencing primer T7_term (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVector ordered from GeneScript.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet15b_ack was a gift from Bernd Nidetzky (Addgene plasmid # 220229 ; http://n2t.net/addgene:220229 ; RRID:Addgene_220229)
