pET21a-TEX264-deltaLRR-His6
(Plasmid #220215)

• Purpose: Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag
• Depositing Lab: Kristijan Ramadan
• Publication: Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.

Backbone

• Vector backbone: pET21a(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TEX264
• Alt name: ZSIG11
• Species: H. sapiens (human)
• Insert Size (bp): 939
• Mutation: c.4-75del / p.2-24del
• Entrez Gene: TEX264 (a.k.a. ZSIG11)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry