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pCDNA5-TEX264-SIM1_mut-cGFP
(Plasmid #220212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Thermofisher
  • Backbone size w/o insert (bp) 5838
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEX264
  • Alt name
    ZSIG11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    939
  • Mutation
    p.111LIDLY>AAAAA; c.331C>G,332T>C,334A>g,335T>C,338A>C,340C>G,341T>C,343T>G,344A>C
  • Entrez Gene
    TEX264 (a.k.a. ZSIG11)
  • Promoter CMV/TetO
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVF_pCDNA3; pCMV_Fw
  • 3′ sequencing primer EGFP_N_Rv
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5-TEX264-SIM1_mut-cGFP was a gift from Kristijan Ramadan (Addgene plasmid # 220212 ; http://n2t.net/addgene:220212 ; RRID:Addgene_220212)

  • For your References section:

    TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts. Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, Ramadan K. Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w. 10.1038/s41467-020-15000-w PubMed 32152270
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