pMRS-BG42-GFP
(Plasmid
#220202)
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Purposemini-Tn7 GFP expression vector - pBG42 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220202 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMRS-GFP
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin and Gentamicin, 50 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemonomeric superfolder green fluorescent protein
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Alt namemsfGFP
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Alt nameGFP
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SpeciesSynthetic
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Insert Size (bp)729
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Mutationmonomeric superfolder green fluorescent protein
- Promoter pBG42
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRS-BG42-GFP was a gift from Larry Halverson (Addgene plasmid # 220202 ; http://n2t.net/addgene:220202 ; RRID:Addgene_220202) -
For your References section:
Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly. Roghair Stroud MN, Vang DX, Halverson LJ. ACS Synth Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312. 10.1021/acssynbio.4c00312 PubMed 39163848