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pCE-BiFC-VC155
(Plasmid #22020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD4983
  • Backbone manufacturer
    Fire lab 1995 kit
  • Backbone size w/o insert (bp) 3969
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VC155
  • Alt name
    Venus(155-238, A206K)
  • Species
    Aequorea Victoria
  • Insert Size (bp)
    255
  • Mutation
    Changed Alanine 206 to Lysine
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Hiatt, S.M., Shyu, Y., Duren, H.M, and Hu, C.D. Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in living C. elegans. Methods, 45:185-191 (2008)

Multicloning sites between HA and VC155 are available

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCE-BiFC-VC155 was a gift from Chang-Deng Hu (Addgene plasmid # 22020 ; http://n2t.net/addgene:22020 ; RRID:Addgene_22020)

  • For your References section:

    Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis. Shyu YJ, Hiatt SM, Duren HM, Ellis RE, Kerppola TK, Hu CD. Nat Protoc. 2008 . 3(4):588-96. 10.1038/nprot.2008.16 PubMed 18388940
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