pSpy
(Plasmid
#220184)
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PurposesgRNA expression vector - promoterless backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 220184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA231
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepromoterless backbone
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gRNA/shRNA sequenceUnspecified
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SpeciesSynthetic
- Promoter promoterless
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSpy was a gift from Larry Halverson (Addgene plasmid # 220184 ; http://n2t.net/addgene:220184 ; RRID:Addgene_220184) -
For your References section:
Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly. Roghair Stroud MN, Vang DX, Halverson LJ. ACS Synth Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312. 10.1021/acssynbio.4c00312 PubMed 39163848