FlhD-GFP
(Plasmid
#220154)
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PurposeTo track the natural FlhD promoter's transcriptional dynamics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAC (Addgene Plasmid #13423)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFlhD promoter only
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SpeciesEscherichia coli
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.11.28.568972v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FlhD-GFP was a gift from Andre Sanches Ribeiro (Addgene plasmid # 220154 ; http://n2t.net/addgene:220154 ; RRID:Addgene_220154) -
For your References section:
A library of reporters of the global regulators of gene expression in Escherichia coli. Dash S, Jagadeesan R, Baptista ISC, Chauhan V, Kandavalli V, Oliveira SMD, Ribeiro AS. mSystems. 2024 Jun 18;9(6):e0006524. doi: 10.1128/msystems.00065-24. Epub 2024 Apr 30. 10.1128/msystems.00065-24 PubMed 38687030