pLX307-VHL(Y98H)-3F-BirA
(Plasmid #220148)

• Purpose: Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB
• Depositing Lab: William Sellers
• Publication: Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038/s41589-024-01590-9.

Backbone

• Vector backbone: pLEX_307 (Plasmid #41392)
• Backbone size w/o insert (bp): 8400
• Total vector size (bp): 10099
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: von Hippel-Lindau tumor suppressor
• Alt name: VHL
• Species: H. sapiens (human)
• Mutation: changed tyrosine 98 to histidine; codon optimized
• Entrez Gene: VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • 3F-BirA (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Weissman Lab (pJW1512, Addgene Plasmid #62366)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry