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Addgene

pRK5-A3-HA-ubiquitin
(Plasmid #220145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220145 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 5020
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin C
  • Alt name
    UBC
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Promoter CMV
  • Tag / Fusion Protein
    • A3-HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Dawson lab (pRK5-HA-Ubiquitin-WT, Addgene Plasmid #17608)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may runs as a dimer (~10kb). Concatenation/Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you need the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-A3-HA-ubiquitin was a gift from William Sellers (Addgene plasmid # 220145)

  • For your References section:

    Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates. Huang HT, Lumpkin RJ, Tsai RW, Su S, Zhao X, Xiong Y, Chen J, Mageed N, Donovan KA, Fischer ES, Sellers WR. Nat Chem Biol. 2024 Mar 21. doi: 10.1038/s41589-024-01590-9. 10.1038/s41589-024-01590-9 PubMed 38514884
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