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Addgene

Cas9-FCPF
(Plasmid #220141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5455
  • Total vector size (bp) 9681
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-FCPF
  • Species
    Synthetic; S. Pyogenes
  • GenBank ID
    WP_010922251.1
  • Entrez Gene
    cas9 (a.k.a. SD90_RS03825, SD90_03830)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas9-FCPF was a gift from Xinlai Cheng (Addgene plasmid # 220141 ; http://n2t.net/addgene:220141 ; RRID:Addgene_220141)

  • For your References section:

    A Chemical Toolbox for Labeling and Degrading Engineered Cas Proteins. Gama-Brambila RA, Chen J, Dabiri Y, Tascher G, Nemec V, Munch C, Song G, Knapp S, Cheng X. JACS Au. 2021 May 12;1(6):777-785. doi: 10.1021/jacsau.1c00007. eCollection 2021 Jun 28. 10.1021/jacsau.1c00007 PubMed 34467332
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