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pET-dCas9-FCPF
(Plasmid #220133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29
  • Backbone size w/o insert (bp) 5049
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dCas9-FCPF
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    3424
  • Mutation
    FCPF sequence inserted on C-terminal
  • GenBank ID
    WP_010922251.1
  • Entrez Gene
    cas9 (a.k.a. SD90_RS03825, SD90_03830)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-dCas9-FCPF was a gift from Xinlai Cheng (Addgene plasmid # 220133 ; http://n2t.net/addgene:220133 ; RRID:Addgene_220133)

  • For your References section:

    Chem-CRISPR/dCas9FCPF: a platform for chemically induced epigenome editing. Altinbay M, Wang J, Chen J, Schafer D, Sprang M, Blagojevic B, Wolfl S, Andrade-Navarro MA, Dikic I, Knapp S, Cheng X. Nucleic Acids Res. 2024 Sep 24:gkae798. doi: 10.1093/nar/gkae798. 10.1093/nar/gkae798 PubMed 39315698
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