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Addgene

pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA7
(Plasmid #220119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI
  • Backbone manufacturer
    addgene
  • Total vector size (bp) 7897
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TadA8e, nSauriCas9
  • gRNA/shRNA sequence
    ctacttAccaaaccatgcctg
  • Promoter EFS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA7 was a gift from Yuxuan Guo (Addgene plasmid # 220119 ; http://n2t.net/addgene:220119 ; RRID:Addgene_220119)

  • For your References section:

    ABE-Mediated Cardiac Gene Silencing via Single AAVs Requires DNA Accessibility. Liu Z, Yang L, Yang Y, Li J, Chen Z, Guo C, Guo Q, Li Q, Zhao D, Hu X, Gao F, Guo Y. Circ Res. 2025 Jan 16. doi: 10.1161/CIRCRESAHA.124.325611. 10.1161/CIRCRESAHA.124.325611 PubMed 39817340
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