pBiFC-VN173
(Plasmid #22010)

• Purpose: Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
• Depositing Lab: Chang-Deng Hu
• Publication: Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Backbone

• Vector backbone: pFlag-CMV
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VN173
• Alt name: Venus (1-172)
• Species: Aequorea victoria
• Insert Size (bp): 516
• Mutation: Venus residus 1-172
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• Articles Citing this Plasmid:
  • 43 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Shyu Y, Suarez C, and Hu, CD. Visualization of ternary complexes in living cells by using a BiFC-based FRET analysis. Nature Protocols, 3:1693-1702 (2008)

Multicloning sites are available between FLAG and VN173