IsHSP70-mCherry
(Plasmid
#220092)
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PurposeExpresses in ISE6 (Ixodes scapularis) cells, heat inducible. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL 4.79 mCherry
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Modifications to backboneRemoved Renilla Luciferase and its associated 3' SV40 polyadenylation sequence from pGL 4.79 [Rluc] (Promega), swapped in mCherry with another SV40 polyadenylation sequence.
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHSP70 promoter from Ixodes scapularis
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SpeciesIxodes scapularis
- Promoter IsHSP70
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RV3 Primer (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.29.569248 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IsHSP70-mCherry was a gift from Monika Gulia-Nuss (Addgene plasmid # 220092 ; http://n2t.net/addgene:220092 ; RRID:Addgene_220092) -
For your References section:
Validation of heat-inducible Ixodes scapularis HSP70 and tick-specific 3xP3 promoters in ISE6 cells. Pham M, Hoffmann HH, Kurtti TJ, Chana R, Garcia-Cruz O, Aliabadi S, Gulia-Nuss M. iScience. 2024 Jul 8;27(8):110468. doi: 10.1016/j.isci.2024.110468. eCollection 2024 Aug 16. 10.1016/j.isci.2024.110468 PubMed 39139404