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Is3xP3-mCherry
(Plasmid #220091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL 4.79 mCherry
  • Modifications to backbone
    Removed Renilla Luciferase and its associated 3' SV40 polyadenylation sequence from pGL 4.79 [Rluc] (Promega), swapped in mCherry with another SV40 polyadenylation sequence.
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis
  • Species
    Ixodes scapularis
  • Promoter Is3xP3

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.29.569248 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Is3xP3-mCherry was a gift from Monika Gulia-Nuss (Addgene plasmid # 220091 ; http://n2t.net/addgene:220091 ; RRID:Addgene_220091)

  • For your References section:

    Validation of a heat-inducible Ixodes scapularis HSP70 promoter and developing a tick-specific 3xP3 promoter sequence in ISE6 cells. Pham M, Hoffmann HH, Kurtti TJ, Chana R, Garcia-Cruz O, Aliabadi S, Gulia-Nuss M. bioRxiv [Preprint]. 2023 Nov 29:2023.11.29.569248. doi: 10.1101/2023.11.29.569248. 10.1101/2023.11.29.569248 PubMed 38076872
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