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Addgene

pNeonGreen-N1-Nir1-613-630
(Plasmid #220088)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNeonGreen-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PITPNM3
  • Alt name
    Nir1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    54
  • Mutation
    amino acids 613-630
  • Entrez Gene
    PITPNM3 (a.k.a. ACKR6, CORD5, NIR1, RDGBA3)
  • Promoter CMV
  • Tag / Fusion Protein
    • GGSGG-NeonGreen (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNeonGreen-N1-Nir1-613-630 was a gift from Gerry Hammond (Addgene plasmid # 220088 ; http://n2t.net/addgene:220088 ; RRID:Addgene_220088)

  • For your References section:

    Nir1-LNS2 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production. Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV. bioRxiv [Preprint]. 2024 Feb 28:2024.02.28.582557. doi: 10.1101/2024.02.28.582557. 10.1101/2024.02.28.582557 PubMed 38464273
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